fgf7 (fibroblast growth factor 7)  (PeproTech)

Journal: Journal of Animal Science and Biotechnology

Article Title: Dietary supplementation with N -acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs

doi: 10.1186/s40104-024-01109-1

Figure Lengend Snippet: The high level of E 2 hyperactivates FGF-FGFR-ERK signaling cascade. A Relative mRNA expression levels of FGFs and their receptors in endometrial explants treated with or without the high level of E 2 ( n = 5). B Immunohistochemical analysis of FGF7 in endometrial explants treated with or without the high level of E 2 . Scale bar: 20 μm. C Western blotting analysis of FGF7 level in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of FGF7 protein level. D and E Western blotting analysis of phosphorylation levels of FGFR ( D ) and ERK ( E ) in endometrial explants treated with or without the high level of E 2 ( n = 7). The upper panel shows the quantification of pFGFR and pERK levels. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. E 2 , estradiol; FGF , fibroblast growth factor; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK

Article Snippet: To further investigate the downstream pathways, explants were cultured in the presence of 200 ng/mL of recombinant fibroblast growth factor 7 (FGF7; Invitrogen), 10 μmol/L FGFR inhibitor BGJ398 (Selleck, Houston, USA), or 100 nmol/L ERK inhibitor PD0325901 (Selleck).

Techniques: Expressing, Immunohistochemical staining, Western Blot

Journal: Journal of Animal Science and Biotechnology

Article Title: Dietary supplementation with N -acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs

doi: 10.1186/s40104-024-01109-1

Figure Lengend Snippet: The high level of E 2 induces overproliferation of endometrium via FGF-FGFR-ERK signaling cascade. A Immunohistochemical analysis of PCNA in endometrial explants treated with or without recombinant FGF7. Scale bar: 20 μm. B Western blotting analysis of PCNA in endometrial explants treated with or without recombinant FGF7 ( n = 7). The upper panel shows the quantification of PCNA level. C and D Western blotting analysis of phosphorylation levels of FGFR ( C ) and ERK ( D ) in endometrial explants treated with or without recombinant FGF7 ( n = 7). The upper panels show the quantification of pFGFR and pERK levels. E and F Western blotting analysis of phosphorylation levels of FGFR ( E ) and ERK ( F ) in endometrial explants treated with the high level of E 2 alone or in combination with FGFR inhibitor ( n = 5). The upper panels show the quantification of pFGFR and pERK levels. G Western blotting analysis of phosphorylation levels of ERK in endometrial explants treated with the high level of E 2 alone or in combination with ERK inhibitor. The upper panel shows the quantification of the pERK level ( n = 5). H Immunohistochemical analysis of PCNA in endometrial explants treated with or without FGFR inhibitor or ERK inhibitor. Scale bar: 20 μm. I Western blotting analysis of PCNA in endometrial explants treated with or without FGFR inhibitor or ERK inhibitor ( n = 5). The upper panel shows the quantification of PCNA level. Data are presented as the mean ± SEM. * P < 0.05, ** P < 0.01. PCNA, proliferating cell nuclear antigen; FGF7, fibroblast growth factor 7; FGFR, fibroblast growth factor receptor; ERK, extracellular signal-regulated kinase; pFGFR, phosphorylated FGFR; pERK, phosphorylated ERK; E 2 , estradiol

Article Snippet: To further investigate the downstream pathways, explants were cultured in the presence of 200 ng/mL of recombinant fibroblast growth factor 7 (FGF7; Invitrogen), 10 μmol/L FGFR inhibitor BGJ398 (Selleck, Houston, USA), or 100 nmol/L ERK inhibitor PD0325901 (Selleck).

Techniques: Immunohistochemical staining, Recombinant, Western Blot

Journal: Avicenna Journal of Medical Biotechnology

Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

doi: 10.18502/ajmb.v16i4.16739

Figure Lengend Snippet: Effect of various treatments on hWJMSCs-Sec toward growth factor proteins level. A) KGF, B) HGF, C) HB-EGF, D) PDGF, E) EGF. The data were presented as mean ± standard deviation. Different superscript letters on Figure A (a, b, c, cd, d, e, f), and B, C, D, E (a, b, c, d, e, f, g) showed significant differences among treatments at p<0.05 (Data was analyzed using ANOVA followed by Tukey HSD post hoc test). Samples included hWJMSCs-Sec (S), Freeze-dried hWJMSCs-Sec (FDS), Freeze-dried Medium Basal (FDMB), Freeze-Dried hWJMSCs-Sec Gel (FDSG), and Freeze-dried Gel Based (FDGB). Number on samples indicate the ratio of gel to hWJMSCs-Sec, 1) 6 g : 3 ml , 2) 6 g : 4.5 ml , and 3) 6 g : 6 ml .

Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

Techniques: Standard Deviation

Journal: Avicenna Journal of Medical Biotechnology

Article Title: The Potential of Human Wharton’s Jelly Mesenchymal Stem Cells Secretome Based Topical Gel for Therapeutic Application

doi: 10.18502/ajmb.v16i4.16739

Figure Lengend Snippet: Proposed mechanism of hWJMSCs-Sec gel as a wound healing agent. * The treatment of hWJMSCs-Sec gel into the wounded skin can regulate some growth factors such as KGF, HGF, HB-EGF, PDGF, and EGF. The upregulation of these growth factors can induce the production of collagen, which plays a crucial role to regenerate tissue formation. The high antioxidant hWJMSCs-Sec gel also has the ability to scavenge free radicals, and inhibit the excessive inflammation that can cause irritation, redness, and pain in the wound.

Article Snippet: The Human KGF (E-EL-H0092), HGF (E-EL-H0084), PDGF (E-EL-H2211), EGF (E-EL-H0059), and HB-EGF (E-EL-H2667) level were assayed using the Elabscience kit, based on manufacturing protocols.

Techniques: